152 THE PRESIDENTIAL ADDRESS. nitrates in pure chalk rock that the supposed sewage contamination might have been derived from antediluvian animals. Experience showed only too clearly, as, for instance, at Caterham, that water might receive the most deadly pollution without appreciably affecting the chemical constitution. Chemistry also failed to explain the purification of rivers after some measure of pollution ; in fact, some chemists denied that purification could take place at all, because water shaken in bottles or running along glass troughs was not purified. A real grasp of the whole problem only became possible when the study of micro-organisms had thrown a light both on the real mode of communication of water-borne disease, and of the changes that go on in water itself. The first attempt in the direction of Bacteriological study applied to water seems to have been made by the late Dr. Angus Smith, who called attention to the great differences shown by samples of water when mixed at a moderate temperature with a sufficient quantity of gelatine to set into a jelly on cooling. Some samples remain for a considerable time without change ; others in a very short time begin to liquefy in more or less numerous points, and very soon show signs of decomposition. Dr. Smith left it, however, to other workers to carry out these investigations with more accurate methods ; and in this country we owe to Dr. Percy Frankland's well-known investi- gations a clear recognition of the importance of this method of the biological study of water. Fuller investigations have developed methods of cultivating the micro-organisms present in water in such a way as to select particular ones from a number of others, and so develop their colonies as to enable the expert to identify them with certainty. Unless this is done we only get the information that there are an alarming number of organisms in the water we drink, which may be very deadly to the human frame, or which may be as harmless as mites in cheese, or, possibly, even beneficial. A very practical result of these researches has been to show the enormous value of the system of filtration through sand which has been adopted by the Water Companies as the result of engineering experience and skill, and which now proves to have excellences the very need for which was not known when the process was worked out. The entire separation of all organisms and germs from water is a most difficult result to obtain by mere filtration ; even with unglazed porcelain, though for a time the liquid passes through sterilized,