348 PREPARATION OF MARINE ANIMALS. be invisible. The Lob-worm, Arenicola, is often much too dark to give good results, but pale specimens, partially dissected, show the entire structure to great advantage, the blood in the chief vessels retaining its natural colour without sensible change. The beautiful purple Nudibranch, Eolis, contains a pigment easily dissolved out by Canada-balsam, but the colouring is retained perfectly for many years, if protected by the animal being well soaked with gum-Arabic, to which a little glycerine has been added to prevent undue brittleness. The Slender Spider-crab, Stenorhynchus, varies much in different localities. Sometimes it is much too dark, but in one year, in the Orwell, it was very pale and the shell comparatively soft, and covered with parasites, which, however, would not have been well seen unless somewhat stained with log-wood, so as to be of about the natural colour. In mounting Crustacea, the principal difficulty is to get rid of the included air, but this may be done by carefully pricking with a sharp, fine needle, when the specimen is in benzole. Priapulus should be killed by fresh water, and kept in it until it has become limp, since otherwise it contracts laterally, and shows little of its proper character. It may also be necessary to slightly stain it. Most excellent preparations may be made by cutting open the body longitudinally, staining it, and spreading it open, so as to show the internal anatomy and the whole muscular structure. It is difficult to prepare good specimens of Cirratulus borealis, since they are often too dark and opaque. My best results have been obtained by killing them in fresh water and leaving them in it until they have swollen considerably, but are not decom- posed. By this means they may be mounted so as to show the remarkably convoluted intestine, and the chief blood vessels filled with red blood. The cirri should be cut off and stained with carmine, so as to be of the natural colour of the living animal, and mounted on the glass after the body has been dried. My specimens of Synapta have been obtained in the Orwell, outside this county. I exhibit them in order to show the great advantage of proper staining. In one specimen the animals were kept for a considerable time in alcohol, with a somewhat diluted solution of madder, so as to penetrate internally. By this means the tentacles, the intestine, the ovaries, and the chief