22 VARIATIONS IN NUMBERS AND HABITAT OF After living on a yacht for some months, it is, of course, desirable to clean off the various animals and plants that have grown on the bottom. Sometimes I can then obtain specimens not otherwise easy to find. The most remarkable circumstance I have noticed is that in different years the animals attached to the bottom may be most strikingly different, though the yacht may have been at the same places, or very nearly so. One year the entire surface under water was covered with small Balani, growing as close to one another as they could ; I estimate that the total number was something like two millions. Another year there were few Balani, but the bottom was covered with Ascidians of the genus Ascidiella. On other occasions the most striking form was Tubularia larynx, good specimens of which I have not been able to obtain in the district except from the bottom of the yacht. In 1899, after lying a long time at Pin Mill, the bottom was covered by soft, tenacious mud, built up by the small Amphipod, Jassa pulchella. On other occasions we have found variable mixtures of the above-named animals. These facts show what great variations there may be from year to year, which is thus so marked, because the bottom of the yacht is always clean to begin with, and animals attached one year cannot be mixed with those attached another year, as must often happen on rocks, and stones, and other natural objects. It will thus be seen that, even in the short period of 10 or 12 years, many noteworthy changes have occurred, and, in some cases at all events, it seems doubtful if the original conditions will be restored in years to come. This is certainly indicated by the fact that in so many cases vast numbers of well preserved dead shells are met with in situ in places where the same species are seldom if ever met with alive. Taking all into account, it certainly appears to me that a considerable number of interesting animals have become more and more rare, whilst but few have become more abundant. The first explanation that suggests itself is that the changes may in some way have been due to human agency, like so many changes in animals and plants inland. In some localities an increase in the amount of sewage discharge may have been the cause; but, though in this respect the worst place I ever stayed at is the water at the back of Walton-on-Naze, yet I never saw a better collecting ground for numerous interesting animals. The use of artificial manures and gas lime may also have had considerable influence in some