10 THE ESSEX NATURALIST. than itself, but occasionally forming almost pure green powdery growths by themselves. The most usual, perhaps, are species of the genus Stichococcus. The patches of incrustation formed by these are usually of a yellower green than those of Pleuro- coccus, and as a rule are found nearer the ground and in more protected situations on the tree trunks. When examined under the microscope the individual cells are seen to be more or less elongated rods, on the average about 1/2500" in length, with the chloroplast going only part of the way round the cell-wall, as in many species of the filamentous alga Ulothrix. The cells, although usually separate, are very frequently joined end to end into short filaments of two or more, and there can be no doubt that the species of this genus are descendants of aquatic Ulothrix types, which have retrogressed towards the unicellular state, possibly during the process of their adaptation to sub-aerial conditions. I pass now to the grey-green incrustation on trees, etc. Although this is produced by a lichen, usually, as I understand, Lecanora varia, I want briefly to refer to it, as the algal cells and fungal hyphae are in such a rudimentary state of association that we can almost regard them as still independent microscopic organisms. The algal cells are rather larger than those of Pleurococcus, being on the average about 1/2000" and they are all nearly spherical. The chloroplast, usually more or less lobed, is placed centrally and it contains a very distinct central body which is probably a pyrenoid. Reproduction, unlike what occurs in Pleurococcus, takes place by the formation within the mother-cell of from four to sixteen aplanospores or autospores, which are provided with their own cell-walls and are only liberated by the rupture or absorption of the wall of the mother-cell. There has been some divergence of opinion in the past as to the genus to which this alga belonged, and it has been referred to Protococcus, Chlorococcum, Chlorella, etc. Recently, however, it has been placed in a special genus, Trebouxia, named by Puymaly after Treboux, who was the first clearly to distinguish its special nature. The same or an exceedingly closely allied form occurs in a great many lichens and possibly all may be regarded as T. humicola Treboux. In the grey-green powdery material on tree trunks, etc., the cells of Trebouxia are nearly all more or less surrounded, either singly or in small groups, by the hyphae of the