THE NANNOPLANKTON OF BOMB-CRATER POOLS. 233 Only small quantities of water are required as a rule for the purpose of centrifuging, about a quarter of a fluid ounce (7 c.c.) being usually sufficient. Even smaller amounts, down to 1 or 11/2 c.c, will often yield quite good samples of nannoplankton, but for centrifuging very small quantities of water specially small tubes fixed on a special head of the centrifuge are required. There seems no need on this occasion to go further into the question of the technique of centrifuging water in order to obtain nannoplankton organisms. Those who may be inclined to follow up the subject will find ample details in a paper already quoted (Lohmann 1911) and in two papers by the present author (Scourfield 1911 and 1930). From what has already been said as to the very small size of nannoplankton organisms it is evident that they can only belong to the unicellular types of plant and animal life, that is to say to the Protophyta and Protozoa. But the surprising thing is that within those great groups they do actually represent so many different types. Thus, in freshwater, in addition to many kinds of Bacteria and a few of Fungi, there are representatives of the Volvocales, Chlorococcales, Conjugates, Xanthophyceae, Chrysophyceae, Bacillariophyceae (Diatoms), Cryptophyceae, Dinophyceae, Euglenineae and Mxyophyceae among the Algae1 and of the Rhizopoda, Heliozoa, Zooflagellata and Ciliata among the Protozoa. As regards the important role played by nannoplankton organisms in the general economy of the sea and fresh waters it may be said to depend upon three principal factors, namely (1) their constant presence in waters of practically every kind, (2) their rapid rate of reproduction under favourable conditions and (3) their general character as "producers," rather than as "consumers," for the great majority, being essentially plants, can synthesise, from inorganic materials, such organic substances as oils, starches, &c. Owing to factors (2) and (3) these minute organisms form the basis of subsistence for many rather larger forms and therefore contribute largely to the foundation of the food-chain which leads from the lowest to the highest forms of aquatic life. Having now, it is hoped, made clear what kind of organisms are included under the term nannoplankton, we may proceed to the immediate subject of the present paper. First of all a few words may be devoted to the bomb-craters and pools themselves, to be followed by the detailed account of the forms of nanno- plankton which have actually been found in them. 1 For classification of the Algae see Fritsch, 1935.