294 THE ESSEX NATURALIST and further work, completed over a number of years would be necessary if these conclusions were to be confirmed. METHODS Specimen Sampling—In two areas, B and G, transect sampling was used. All other samples taken were random samples. Each sample covered an area of 100 sq. cms. to a depth of 15 cms. The figures in Table IV are obtained from two such samples at each station. Water Content—A core sample of the substratum at each station was obtained by using a glass tube 5/8th of an inch in diameter and 10 inches long. This was screwed into the sub- stratum and a rubber bung inserted in the top of the tube. The tube was then screwed out again and another bung inserted in the other end, Fraser (1932), Stopford (1951), Southward (1953). The water content of one inch of the core from half inch below the surface was estimated in the normal way. Soil Analysis—Core samples were obtained in the same way as above, air dried and analysed later in the laboratory. The method described by Rees (1940) was found to be unsatisfactory and a more refined method, in which subsamples are taken from a soil suspension by pipette, was therefore used. This method will be described in detail in another paper. Salinity—The amount of sodium chloride was estimated by the usual method of titrating N/10 silver nitrate against 10 mls. of seawater to which a few drops of potassium chromate had been added. Total salt content was obtained by consulting Hydro- graphical tables—Knudsen (1901—reprint 1953). Salinity PHYSICAL AND CHEMICAL DATA TABLE I The above table shows that there is little variation in the salinity of the water with distance up the Creek, just over 1‰, at low water and just less than 1‰ at high water. Benfleet Creek is therefore not a true estuary in that there is no real gradation in salinity between source and mouth. All the salinities, however, are below the full North Sea salinity of 33‰ (Westcliff Sea Front being the nearest to this) as a result of the infiltration of fresh water from the Thames Estuary.