ECOLOGICAL STUDIES IN SOUTH BENFLEET CREEK 295 pH The pH at low water in Benfleet Creek is: — 7.7 on the Transect in Area G—Benfleet Bridge 7.6 on the Transect in Area B—Leigh Island Time did not permit a complete range of samples to be taken, for example from transect in area B but a number of points are indicated by Table II. Water content of the substratum is dependent on three major factors: — 1. Period of exposure between tides. 2. Distribution of particle size. 3. Efficiency of packing. And on two minor factors: — 1. Climatic conditions at the time of sampling. 2. Degree of slope. If on each of the transects, i.e. in Area B and G, particle size and efficiency of packing were the same then the water content would increase towards low tide. This is suggested in Transect B. The slight variations from this gradation can be explained in terms of the differences in substrate composition. There is much greater variation in water content in the transect in area G, and here all three factors are important. It may be concluded that the water content of stations 2 and 3 is a reflection of particle size, or soil composition. The result obtained for station 1 is very high because it is only a few yards above low water mark and is therefore only exposed for a short period. The result for station 4, on the other hand, is very low because at the time of sampling the station had not been covered for a few days by the Neap Tides. As a result of this, and the climatic conditions for the period August 24-31st (approx. period of Neap Tides) the surface had almost completely dried out and was cracked and sunbaked. Random samples were taken in areas A, B and E in order to give an indication of the water content of these areas, and the same applies for the Long Transect.