A METHOD OF QUALITATIVE BIOLOGICAL RIVER SURVEY 251 of only 0.5° C. The water may be regarded as "hard". For comparison "moderate" hardness is 110-150 p.p.m. CaC03 while "soft" waters are up to 90 p.p.m. CaCO3. The potassium content is about average for Essex rivers. What is somewhat surprising is that the river water at the sampling station contained no phosphate, despite the sewage disposal works discharges upstream. One can only conclude that this was either removed by aquatic plants or the phosphate contribution from the sewage works was negligible on the day of sampling. It is known that the phosphate content of sewage effluent can vary consider- ably from day to day. The chloride ion concentration is below average for Essex rivers, but large fluctuations are possible due to sewage disposal works discharges. This ion normally remains unchanged on entry to river water, its concentration decreasing by dilution rather than by plant and animal utilization or physical absorption or adsorption on organic or inorganic surfaces. From the above chemical results it is concluded that there is little chemical evidence of organic pollution. The B.O.D. is average for clean river water. The oxygen demand is low and this shows the river to have recovered satis- factorily from previous organic pollution. The D.O. is high and thus the available oxygen for aquatic animals is adequate. While the temperature is rather high, 15°C (60°F) it would have to rise to 25 C (78°F) or above to affect the aquatic animals of the habitat to any observable extent. It is uncommon for Essex rivers under dry weather flow conditions to reach or even exceed 20°C in Summer. Some cooling of the river water over the riffle is evident, but is not significant. Biologically the calcium concentration of the river water at this sampling station is very high. According to the snail criterion of hardness, water becomes soft at or below 20 p.p.m. which shows that the calcium concentra- tion of the habitat contains sufficient of this ion to meet the needs, not only of the snail population, but also of all other organisms present. The pH of 8.1 is the value normally to be expected of Essex rivers except in cases of gross organic and trade effluent pollution, when it can drop to pH 5 or 6, but rarely below this value, since river water is a very well buffered system. A pH of 5 is not unfavourable to the aquatic life of a riffle. In conclusion, the chemico-biological interpretation of the analyses, suggests that the river is clean and unpolluted. BIOLOGICAL ASSESSMENT OF THE RIFFLE HABITAT The methods adopted for the estimation of the larger aquatic vertebrates and invertebrates are already well known to biologists. I have tried to standardise these methods, for while the principle of collection may be the same, many variations are possible. This