A METHOD OF QUALITATIVE BIOLOGICAL RIVER SURVEY 259 others. The biological response of macro-aquatic organisms is generally the same and may be conveniently summarised diagrammatically in Fig. 7, in which the curves are adapted from Bartsch (1948), although it is not known what sampling techniques or places sampled were used in their production. This shews three main biological zones which represent respectively areas of gross, moderate and light organic pollution. The curves shew that if the pollution is merely moderate then immediately below the source of polluting discharge the tubificid zone would be absent and the first zone would be that of the chironomids. It is at once evident that the zone of recovery studied in this paper is that represented by the chironomids with the degree of overlap of distribution curves varying with the type of organic pollution, its intensity and the station selected for sampling, etc. It would seem that similar curves could be plotted for Erpobdella and Nemacheilus. Fig. 8. Possible food-web in the riffle habitat studied