296 THE ESSEX NATURALIST Method I. In the field To ensure that the survey at Wrabness would be based upon accurately recorded information, each pair of students was encouraged to collect and label representative specimens that were subsequently used for reference. This essential preliminary work was carried out in an area well away from the site selected for detailed study and was accompanied by a brief description of the salt-marsh and its flora. Meanwhile, extending across the marsh and passing through several features of interest, a line of bamboo canes was set up, each of which was carefully lined-up with the tower of Royal Hospital School clearly visible across the estuary. At the point where the line passed on to the sea wall an iron pipe was driven into the ground. Although this pipe is at the origin referred to in Fig. 2 and, together with the tower, identifies the line, an arbitary datum on the general marsh served as a more convenient zero point by which each sample was located. String was used to indicate the line, and measuring tapes, pulled taut to avoid following the surface contours, were held in position by fastening them to the bamboo canes. The sampling began with the first pair of workers placing their quadrat on the ground, one edge in contact with the string and one corner at the zero point. The second pair laid their quadrat on the same side of the line and with the corresponding corner at the 3-feet mark. The next pair began at the 6-feet mark and so on down the line. This is illustrated in Plate 3. A separate proforma, indentified by the position of the quadrat (distance in feet "above" or "below" the zero point) and by the names or code letter of the workers concerned, was used for each and every sample. Two values were recorded against the name of each species rooted within the quadrat. (a) The number of smaller squares (0-25) in which the species was present. (b) The average number of plants in each smaller square (i.e., the total number of plants divided by 25). For plants such as Puccinellia maritima it was impracticable to count the num- ber of tillers and no estimate of density was made. Where necessary, the names of species not already listed were added to the proforma and if no plants were present within the quadrat, additional information (e.g., bare mud, water-filled channel or path) was included by way of explanation. The completed proforma was then handed in to the person in charge who checked it for obvious errors and omissions. The next unsampled position at the end of the double row of workers was then allocated, a fresh proforma issued and the sampling procedure repeated. By this means, a transect 840 feet long was sampled at 3-feet intervals by twelve pairs of students in less