188 THE ESSEX NATURALIST Pymmes Brook rarely exceeds 20 million gallons per day and (b) the sewage effluent entering Pymmes Brook is only treated to the Royal Commission standard of 30 p.p.m. and 20 p.p.m. for sus- pended solids and biological oxygen demand, respectively, there is insufficient dilution in the Lee and a serious deterioration in water quality occurs immediately downstream. Table 2 shews the chemical quality of the River Lee at about 75 metres upstream of the confluence with Pymmes Brook and these figures, par- ticularly those for dissolved oxygen, should be compared with the Hackney results. Since the poor quality of the water produces a barrier for many species the effect on the fauna and flora is con- siderable but it may have aided the establishment of the viable guppy population since other fish, save Three-spined Stickleback, Gasterosteus aculeatus, can only rarely penetrate into the Lee as far downstream as Hackney. Throughout most of its length in the. lower reaches the River Lee Navigation is about 19 metres wide with vertical sides and a maximum depth of about 2.5 metres. At all times of the year the river bed at Hackney contains extensive deposits of decompos- ing organic mud and this is anaerobic below 2 cm depth. The bed provides optimum conditions for vast numbers of oligochaet worms, which may occur at densities greater than 20,000 individuals