Their Collection and Investigation. 51 unassisted eye, but had a diameter or total length of as much as from the one-tenth to the half of an inch. The inhabitants of these relatively large tree-like or sac-shaped fabrications in no case yielded a greater measurement than the l-4000th or the 1-3000th part of an English inch, so that the number included in the larger aggregates is almost beyond calculation. The student must by no means confine himself to the collection and examination of water-plants in his search for Infusoria. Innumerable forms are to be met with growing upon other aquatic animals : insect larvae, molluscs, and the more minute Crustacea, yielding an abundance of different forms, their "fluffy" appearance, as seen with the naked eye or with the aid of a lens, being a sure sign of the presence of the required organisms. The little Entomostracan— Cyclops—may be more especially mentioned as commonly supporting on its body and limbs a perfect forest-like growth of infusorial types, including, it may be, representatives of each of the three primary sections referred to in my earlier observations. In this manner the class Ciliata is usually typified by one or more species of Epistylis; that of the Flagellata by Cephalothamnium and Deltomonas; while that of the Tentaculifera may be represented by one or two species of Podophrya; and all of which Infusoria, as so far known, are obtained under no other conditions. Although ponds, ditches and other expansions of water may be recommended to the student as his first area of exploration, these represent but one out of numberless sources from whence Infusoria are to be derived. A multitude of species are essentially parasitic, living within the intestinal viscera of higher animals. Many terrestrial insects, worms, and molluscs, discharge the duty of hosts to special varieties. The frog, in common with other Amphibia, supports quite an abundant infusorial fauna, consisting of both ciliate and flagellate types ; while even the higher Vertebrata, including horses, sheep, oxen, and man himself, are not exempt from infusorial dependents.2 2 [An article descriptive of the chief varieties of parasitic Infusoria has been contributed by the author to the 'Popular Science Review' for 1880, p. 293.—Ed.