52 Infusoria: What are they ? Artificial animal and vegetable macerations yield very numerous forms, and are especially rich in the flagelliferous Monads. Up to a comparatively recent date the derivation of the Infusoria produced so abundantly in hay infusions has been a marked subject of contention among physiologists, some arguing that they were generated there spontaneously, and others that they were developed from extraneously derived pre-existing germs. By carefully conducted experi- ments it has been my good fortune to successfully demonstrate not only that the germs or spores of Infusoria abound in hay previous to maceration, but also that the Infusoria which produced these germs flourish in the living condition in the grass previous to desiccation, remaining in a state of temporary encystment during dry weather, and reawakening to life with each shower of rain or fall of dew.3 A few concluding suggestions respecting the collection and observation of Infusoria may not prove unacceptable. From whatever sources samples of water containing specimens are derived they should be kept separate, and have a label indi- cating the place and date of collection affixed. Not only are species mingled indiscriminately prone to prey upon each other, but rare varieties may appear upon the scene whose precise habitat cannot be successfully traced after such admixture of the material collected. The student is advised to habituate himself to make drawings, however rough, of every species he encounters, placing by the side of each sketch a registration of its precise dimensions, taken with the aid of au eye-piece micrometer. The essential structural points that he should further seek to verify are the position and character of the oral and excretory apertures, and similar data with reference to both the contractile vesicle and the nucleus or endoplast if distinctly developed. The nature and distribution of the cilia or other appendages will, of course, receive primary attention; the precise number and 3 [The whole question of biogenesis versus abiogenesis is fully discussed in chap. iv. of the 'Manual of the Infusoria'; and Mr. Kent's origins and valuable observations, above alluded to, are detailed at length at page 140, et seq., of the same work.—Ed.]